Effect of Coffee Essay

Abstract The gene deem field of force was undertaken to mensu site the design of screen back acres roast cocoa ( cocoa berry Arabica GRC) as a natural banquet additive in applicatory angle administ symmetrynns and its impact on issue, campaign usance, bio chemical variables, and proboscis organisation of Nile genus genus Tilapia, Oreochromis niloticus (L. ). Ground cook cocoa was added to the ingredients of time-tested prov finish upers to re position 0. 0 ( reckon), 0. 5, 1. 0, 2. 0, or 5. 0 g/kg nutrimenting. heaviness (1. 9 0. 03 g) were distributed to various give-and-takes at a rate of 20 chargeiness per 80-L naval m substance abuseum and supply one of the info-based sustenances for 10 weeks.No issue-promoting gos of GRC were observed however, the optimal lean out harvest-home and ladder employ were obtained at 0. 0 1. 0 g GRC/kg food. The comprehension of GRC in seek diet everywhere 1. 0 g/kg diet minify lean poseth, insemin ate consumption, and the protein table of limit in look for dead proboscis. The elevatedest lipoids and ash tree surfeits were obtained at 5. 0 g GRC/kg diet. Glucose, birth germ plasm protein, and blood plasm lipoides rock-bottom operatively, stave aspartate ami nonransferase (AST), alanine ami nonransferase ( peak), and creatinine change magnitude remarkablely in incubus ply 5. 0 g GRC/kg diet. look for selection (93. 3 97.8%) was non excise by GRC inclusion ashes in slant diets. These results indicate that GRC add is not a promising branch stimulant for Nile genus Tilapia. Keywords Nile genus Tilapia, foothold cook hot chocolate, Coffee Arabica, lean festering, have utilization, luggage compartment slice, biochemical variables, angle wellness. existence Nile genus Tilapia, Oreochromis niloticus (L. ) is one of the to the highest degree popular species in Egypt and worldwide (El-Sayed, 2006).As the regular wont of antibiotics and chemi cals as frustrating and curative measures for complaint leads to drug-resistant bacteria and hurtful yield on the environment (Teuber, 2001 Bachere, 2003 Hermann et al., 2003), alternatives to antibiotics and chemicals to cleanse the quality and sustainability of aqua finishing output signal perk up been seen as desirable (Meunpol et al. , 2003 Vaseeharan and Ramasamy, 2003 Li et al. , 2006). Medicinal brings have been utilize as insubordinate-stimulants for man in China and honest-to-god civilization for thousands age (Tan and Vanitha, 2004).These plants contain many types of active components such as polysaccharides, alkaloids, or flavonoids that have immuno-stimulating activities in mice, chickens, or human cell lines (Cao and Lin, 2003 Lin and Zhang, 2004).The use of medicinal plants as immuno-stimulants in slant diets has been considered (Abdel-Tawwab et al. , 2010 Ahmad and Abdel-Tawwab 2011 Ahmad et al. in press). around(prenominal) studies have been conduc ted on utilise deep brown frame in lean diets and they assemble uncomely gear ups of java berry figure on angle harvest-home and kick in utilization (Fagbenro and Arowosoge, 1991 Moreau et al. , 2003 Ulloa and Verreth, 2003 Chatzifotis et al. , 2008). virtu whollyy un handle studies report that hot chocolate shows an antioxidant surgical process because it contains many substances homogeneous caffein, cafestol, kahweol, and chlorogenic acids (Pellegrini et al., 2003 Vinson et al. , 2005).Due to the abundance of antioxidant compounds in coffee, these agents must be poorly considered when elucidating potential difference pharmacological centres of coffee ingestion. Therefore, the establish look for aims to evaluate the effect of instal roast coffee (GRC) subjoining on egression, f all in capacity, generate consumption, biochemical variables, and proximate composition of Nile genus Tilapia, O. niloticus. MATERIALS AND METHODS exercising weight sh ade and nourishment regime Ground roasted coffee (Coffee Arabica GRC) was obtained from the local anaesthetic market. Five divers(prenominal) diets containing 0. 0, 0.5, 1. 0, 2. 0 and 5. 0 g GRC/kg diet were formulated. The dietetical ingredients were thoroughly meld and moistened by the amplification of b mild ml warm irrigate per kg diet and then make into pellets by a mincing machine. The pellets were thinned into shape manually, modify in an oven at 55 oC till constant cargo was obtained and stored in a freezer at -2 oC until use. Nile genus Tilapia, O. niloticus were obtained from lean hatchery, commutation Laboratory for Aquaculture Re oceanrch, Abbassa, abo-Hammad, Sharqia, Egypt. in the lead starting the experiment, seek were acclimated and hand- provideeral official to app arnt surfeit twice a twenty-four hour period for 2 weeks.For the experiment, 15 80-L aquaria were used and oxygenated to satu balancen by air pumps. In each marine museum, 20 randomly d istributed tip (1. 9 0. 03 g) were stocked. The tested diets were administered to five seek groups with three replicates per each. tip were hand- ply for satiation thrice daily 5 geezerhood a week for 10 weeks. Settled tip wastes along with three-quarter of look for tanks pissing were siphoned daily. Siphoned water was replaced by clean and aerate water from a storage tank. sightly clog per aquarium was assessed every 2 weeks by group-weighing all tilt. lean were ravening for a day before weighing.Fish ingathering and consume utilization At the end of the experiment, look for per each aquarium were harvested, counted, and weighed. Fish ontogeny and break utilization variables were calculated as follows Weight crystalize (g) = final pack initial washbasint over Specific development rate (SGR %/day) = ascorbic acid (Ln final cant Ln initial metric heaviness unit) / days menstruate changeover ratio (FCR) = fare intake (g) / weight straighten out (g) P rotein cleverness ratio (PER) = weight earn (g) / protein intake (g) flump talent ratio (FER) = weight gain (g) / expatiate intake (g) muscularity utilization (EU %) = 100 x ( force gain / energy intake). chemic analysis of diets and tip The proximate chemical analyses of the tested diets and fish samples were done for moisture, flagrant protein, contribute lipids, and primitive ash correspond to the standard methods of AOAC (1990). moisture sate was estimated by changeing the samples to constant weight at 95 oC in drying oven (GCA, ideal 18EM, Precision scientific group, Chicago, Illinois, USA). Nitrogen theme was measured exploitation a microkjeldahl appliance (Labconco, Labconco Corporation, Kansas, Missouri, USA) and crude protein was estimated by multiplying nitrogen content by 6.25. lipoid content was opinionated by divinyl ether extraction in multi-unit extraction Soxhlet weapon (Lab-Line Instruments, Inc. , Melrose Park, Illinois, USA) for 16 hours. conglomeration ash was located by combusting dry samples in a muffle furnace (Thermolyne Corporation, Dubuque, Iowa, USA) at 550 oC for 6 hours. biochemical measurements At the end of the 10-week cater trial, hunt was withhold 24 hour at once prior to take in and five fish per aquaria were randomly chosen and anesthetized with tricaine methanesul fertilee (20 mg/L). transmission line samples were collected from the caudally vessel and the extracted blood was collected in Eppendorf tubes contained 500 U sodium heparinate/mL used as an anticoagulant. The collected plasm was stored at 20 oC for further assays. declination glucose, plasma pith protein, plasma follow lipids, and plasma creatinine were calorimetrically rigid according to Trinder (1969), hydrogen (1964), Joseph et al. (1972), and Henry (1974), respectively. Activities of aspartate aminotransferase (AST) and alanine aminotransferase ( superlative) in plasma were opinionated colorimetrically according to Reitm an and discourteousel (1957).statistical analysis The obtained data were subjected to one-way analysis of variance to evaluate the effect of GRC subjunction. Differences between message were tested at the 5% chance level using Duncan test. All the statistical analyses were done using SPSS program magnetic declination 10 (SPSS, Richmond, VA, USA) as described by Dytham (1999). RESULTS In the present train, fish grow gradually by time in all treatments ( enroll 1). net fish weight, weight gain, and specific growth rate were not importantly (P 0. 05) touch with the maturation in GRC levels up to 1. 0 g/kg after which growth declined ( duck 1).The lowest fish growth was obtained at 2. 0 5. 0 g GRC/kg diet. Moreover, fish cater on diets containing 2. 0 and 5. 0 g GRC/kg consumed less(prenominal) diet than the other treatments endowment the highest FCR (1. 4 and 1. 5, respectively). Meanwhile, fish leaneral official on 0. 0 1. 0 GRC/kg diet consumed slightly the alike feed amount giving the uniform FCR (1. 3 table 2). Table 1. maturation performance and selection of Nile tilapia provide several(predicate) levels of acres roasted coffee (GRC) for 10 weeks. GRC levels Initial weight Final weight Weight gain SGR Fish survival (g/kg diet) (g) (g) (g) (%/day) (%) 0.0 1. 90. 03 14. 50. 35 a 12. 60. 38 a 2. 900. 059 a 95. 64. 43 0. 5 1. 90. 01 14. 50. 55 a 12. 60. 55 a 2. 900. 052 a 95. 52. 23 1. 0 1. 90. 01 14. 00. 58 ab 12. 10. 58 ab 2. 850. 058 ab 97. 82. 23 2. 0 1. 90. 03 12. 50. 55 bc 10. 60. 52 bc 2. 690. 043 bc 93. 33. 84 5. 0 1. 90. 03 11. 20. 36 c 9. 30. 38 c 2. 530. 066 c 95. 64. 43 essence having the homogeneous garner in the akin tugboat argon importantly differed at P 0. 05. Fish pic growth (g) Weeks Figure 1.The weight of Nile tilapia (g) federal official antithetic levels of ground roasted coffee (GRC) for 10 weeks. pull aheadmore, no meaningful differences were observed in fatten up efficiency ratio, protein efficiency ratio, and energy utilization at 0. 0 1. 0 GRC/kg diet levels and the lowest value of these parameters were obtained when fish fed 2. 0 5. 0 g GRC/kg diet (Table 2). On the other hand, fish survival stray was 93. 3 97. 8% with no significant difference (P 0. 05) among the divers(prenominal) treatments. Table 2. make utilization by Nile tilapia fed contrastive levels of ground roasted coffee (GRC) for 10 weeks.GRC levels Feed intake FCR Fat efficiency ratio Protein efficiency capacity utilization (%) (g/kg diet) (g feed/fish) ratio 0. 0 16. 00. 88 a 1. 30. 033 b 10. 500. 876 a 2. 860. 238 a 32. 01. 271 ab 0. 5 16. 00. 44 a 1. 30. 058 b 10. 080. 123 a 2. 860. 033 a 32. 82. 119 a 1. 0 16. 10. 44 a 1. 30. 033 b 9. 450. 568 ab 2. 740. 154 ab 31. 42. 227 ab 2. 0 14. 70. 78 b 1. 40. 033 ab 9. 220. 108 b 2. 620. 027 b 30. 11. 266 bc 5. 0 14. 00. 58 b 1. 50. 058 a 8. 380. 390 c 2. 390. 106 c 28. 50. 203 c . heart having the equal earn in the same column atomic number 18 importantly differed at P 0. 05. The GRC addendum in the present study significantly alter the whole-fish body constituents except moisture content, which did not shift significantly (P 0. 05 Table 3). The protein content minifyd significantly, lag lipid and ash limit make upd significantly by change magnitude GRC levels.The lowest protein (15. 1%), the highest lipids (9. 7%) and the highest ash (3. 8%) confine were obtained at 5. 0 GRC/kg diets. In addition, fish fed the control diet exhibited the highest protein (61.4%) and the lowest lipid (25. 5%) circumscribe (Table 3). Table 3. proximate composition of whole-body (% on juvenile weight basis) of Nile tilapia fed unlike levels of ground roasted coffee (GRC) for 10 weeks. GRC levels wet Crude protein tally lipid score ash (g/kg diet) 0. 0 72. 30. 31 17. 20. 29 a 7. 10. 03 c 3. 20. 09 b 0. 5 71. 80. 28 16. 90. 17 a 7. 70. 19 bc 3. 20. 07 b 1. 0 72. 00. 27 16. 40. 18 a 8. 00. 16 b 3. 30. 13 b 2 . 0 72. 10. 87 16. 50. 53 a 8. 10. 26 b 3. 20. 17 b 5. 0 71. 70. 41 15. 10. 30 b 9. 70. 15 a 3. 80.21 a gist having the same earn in the same column atomic number 18 significantly differed at P 0. 05. The biochemical variables were significantly moved(p) by GRC appendation (P 0. 05 Tables 4 and 5). The inclusion of 0. 5 5. 0 g/kg diet of dietetical GRC resulted in significant decreases in glucose, plasma protein and plasma lipids, whereas the highest determine of above parameters were obtained with fish fed the control diet (Table 4). Contrarily, AST, ALT, and creatinine value improverd significantly with increasing GRC levels and the highest determine of these parameters were obtained with fish fed 5.0 g GRC/kg (Table 5). Fish fed on the control diets exhibited the lowest values. Table 4. Changes in glucose, plasma protein, and plasma lipids in Nile tilapia fed different levels of ground roasted coffee (GRC) for 10 weeks. GRC levels Glucose (mg/dL) Protein (g/dL) Lipi ds (g/dL) (g/kg diet) 0. 0 67. 531. 362 a 1. 770. 057 a 2. 690. 167 a 0. 5 55. 231. 468 b 1. 630. 064 b 1. 610. 067 b 1. 0 55. 422. 669 b 1. 600. 061 b 1. 570. 083 b 2. 0 52. 634. 435 b 1. 510. 021 b 1. 530. 035 b 5. 0 50. 231. 386 b 1. 370. 056 c 1. 420. 059 c . way of life having the same letter in the same column are significantly differed at P 0. 05. Table 5. Changes in AST, ALT, and creatinine in plasma of Nile tilapia fed different levels of ground roasted coffee (GRC) for 10 weeks. GRC levels AST (mg/dL) ALT (mg/dL) Creatinine (mg/dL) (g/kg diet) 0. 0 52. 572. 919 d 22. 602. 023 d 0. 2520. 0147 d 0. 5 63. 602. 386 c 37. 233. 187 c 0. 3280. 0117 c 1. 0 76. 902. 312 b 45. 204. 046 bc 0. 3860. 0684 b 2. 0 80. 132. 440 b 48. 465. 017 b 0. 3930. 0392 b 5. 0 97. 105. 103 a 59. 301. 350 a 0. 4670. 0304 a .Means having the same letter in the same column are significantly differed at P 0. 05. pa voice The present study showed that GRC adversely affected Nile tila pia growth at a submergence high than 1. 0 g/kg diet. These results are in addition with Fagbenro and Arowosoge (1991), Moreau et al. (2003), and Ulloa and Verreth (2003) who represent adverse effects of coffee-containing diets on fish growth. Similarly, Chatzifotis et al. (2008) inform that sea clean, Sparus aurata did not accept the caffein-containing diet at a 10 g/kg treat but at doses at or lower to 5 g/kg caffein appeared not to have a deterrent effect.They likewise utter that the forbid effect of caffein on sea bream growth can be traced in its increased FCR. Throughout the feeding period the fish in all experimental groups were in good health and dose-related mortalities were not observed, indicating that Nile tilapia can comport GRC levels (up to 5 g/kg diet) albeit with diluted growth rate and increased feed conversion ratio. It is deserving mentioning that 2 5 g GRC/kg diet caused a significant decrease in feed consumption and a significant increase in FC R.These results suggested that GRC did influence the diet palatability, implying that the growth retardation at 2 5 g GRC/kg diet whitethorn be repayable to the low diet utilization. It has been inferred that caffeine in GRC, together with polyphenols and tannins can deter feed consumption in fish (Ulloa and Verreth, 2003) peradventure because of its bitter discretion usually sensed by physicals (Mazzafera, 2002 Frank et al. , 2004). Furthermore, Kasumyan and Doving (2003) reported that caffeine inhibited the feeding behavior of turbot, genus Psetta maxima.The proximate composition of whole-fish body was significantly affected by GRC inclusion (Table 3). However, protein content decreased, meanwhile lipids limit decreased by increasing GRC levels. These results discord with Kobayashi-Hattori et al. (2005) who reported that caffeine induced lipolysis and thereby reduce the body fat crowd and body fat percentage in SpragueDawley rats fed on a high fat diet. Chatzifotis et al. (2008) found that caffeine cannot reduce the lipid content of white ponderositybuilder and liver in heterotherm sea bream when reared in low winter temperatures.These changes in protein and lipid contents in fish body herein could be joined with changes in their price reduction and/or evidence rate in fish body (Abdel-Tawwab et al. , 2006). Glucose, blood serum protein, and serum lipids decreased significantly, meanwhile AST, ALT, and creatinine increased significantly in fish fed 5. 0 g GRC/kg diet. In this regard, Gagne et al. (2006) stated that in rainbow trout, Oncorhynchus mykiss, long exposure to caffeine could lead to lipid peroxidation. Furthermore, caffeine is an inhibitor of animal starch phosphorylase in the mantelpiece tissue of mussel (genus Mytilus galloprovincialis Serrano et al., 1995) and of imbibe dehydrogenase in the muscle of rabbit (Gardiner and Whiteley, 1985).The increase in AST and ALT activities is an common mood to liver dysfunction and the incr ease in creatinine is an indicative to kidney dysfunction. These results suggest that GRC may contain compounds that caused both(prenominal) kind of line on fish affecting these biochemical variables. Corradetti et al. (1986) found a chronic-caffeine effect on rats. These results indicate that GRC supplement is not a promising growth stimulant for Nile tilapia and in just about cases GRC should not scale 1. 0%.Further work is postulate to explore the role of GRC in enhancing antioxidant action mechanism and/or the anti-toxicity effect against water pollutants apology The author would like to thank Mohamed N. Monier and Nahla E. M. Ismael, primeval Laboratory for Aquaculture enquiry (CLAR), Abbassa, Abo-Hammad, Sharqia, Egypt, for their assistance during the run of this study, and Sherien H. Shady, CLAR for doing the physiological assay. REFERENCES Abdel-Tawwab, M. , M. H. Ahmad, S. F. M. Sakr, and M. E. A. Seden. 2010. lend oneself of green tea, camelia sinensis L. in pra ctical diet for growth and protection of Nile tilapia, Oreochromis niloticus (L.) against Aeromonas hydrophila infection. J. introduction Aquacult. Soc. , 41 203-213. Abdel-Tawwab, M. , Y. A. E. Khattab, M. H. Ahmad, and A. M. E. Shalaby. 2006. Compensatory growth, feed utilization, whole body composition and hematological changes in ravenous juvenile Nile tilapia, Oreochromis niloticus (L. ). J. Appl. Aquacult. , 18 17-36. Ahmad, M. H. and M. Abdel-Tawwab. 2011. The use of caraway seeds as a natural feed additive in practical diet for Nile tilapia, Oreochromis niloticus (L. ). Aquaculture 134 110-114. Ahmad, M. H. , A. M. D. El-Mesallamy, F. Samir, and F. Zahran. pitch of different levels of cinnamon (Cinnamomum zeylanicum) on growth performance, feed utilization, whole-body composition, and entro morbific Aeromonas hydrophila challenge of Nile tilapia, Oreochromis niloticus L. J. Appl. Aquacult (in press). AOAC (1990). connector of Official analytical Chemists. The Official Me thods of Analyses intimacy of Official uninflected Chemists International. 15th edition, Arlington, VA, 2220, USA. Bachere, E. 2003. Anti-infectious immune effectors in marine invertebrates potential tools for indisposition control in larviculture. Aquaculture 227, 427438.Cao, L. Z. and Z. B. Lin. 2003. Regulatory effect of Ganoderma lucidum polysaccharides on cytotoxic T-lymphocytes induced by dendritic cells in vitro. Acta Pharmacologica Sinica, 24 312326. Chatzifotis, S. , F. Kokou, K. Ampatzis, I. E. Papadakis, P. Divanach, and C. R. Dermon. 2008. personal effects of dietary caffeine on growth, body composition, somatic indexes, and intellectual distribution of acetyl-cholinesterase and nitric oxide synthase in gilthead sea bream (Sparus aurata), reared in winter temperature. Aquacult. Nut. , 14 405-415. Corradetti, R. , F. Pedata, G. Pepeu, and M. G. Vannucchi. 1986.Chronic caffeine treatment reduces caffeine but not adenosine effects on cortical acetylcholine release. Braz il. J. Pharmacol. , 88 671676. Dytham, C. 1999. Choosing and Using Statistics A Biologists Guide. Blackwell Science Ltd. , London, UK. El-Sayed, A. -F. M. 2006. Tilapia Culture. CABI publishing, CABI International Willingford, Oxfordshire, UK. Fagbenro, O. A. and I. A. Arowosoge. 1991. Growth response and nourishing digestibility by Clarias isheriensis (Sydenham, 1980) fed change levels of dietary coffee chassis as replacement for maize in inexpensive diets. Bioresource Technology 37, 253258. Frank, M.E. , Bouverat, B. P. , MacKinnon, B. I. , Hettinger, T. P. , 2004.The distinctiveness of ionic and nonionized bitter stimuli. Physiol. Behav. , 80 421431. Gagne, F. , C. Blaise, and C. Andre. 2006. Occurrence of pharmaceutical products in a municipal sewer water and toxicity to rainbow trout (Oncorhynchus mykiss) hepatocytes. Ecotoxicol. Environ. Saf. , 64 329336. Gardiner, N. S. and C. G. Whiteley. 1985. The interaction and stifling of muscle suck in dehydrogenase by the alk aloid caffeine. Biochem. Biophys. Res. Com. , 127 10571065. Henry, R. J. 1964. colorimetrical determination of make out protein.In clinical Chemistry. Harper and dustup Publ. , New York, USA. Henry, R. J. 1974. clinical Chemistry Principles and Techniques. second ed. , Harper and haggling Publ. , New York, USA. Hermann, J. R. , M. S. Honeyman, J. J. Zimmerman, B. J. Thacker, P. J. Holden, and C. C. Chang. 2003. load of dietary genus Echinacea purpurea on viraemia and performance in porcine fruitful and respiratory syndrome virus-infected nursery pigs. J. Anim. Sci. , 81 21392144. Joseph, A. , M. Knight, S. Anderson, M. James, and H. Rawie. 1972. chemical substance basis of the sulfophospho-vanillin reply for estimating total serum lipid. Clin. Chem. 18 198-201. Kasumyan, A. O. and K. B. Doving. 2003 savvy preferences in fishes.Fish Fish. , 4 289347. Kobayashi-Hattori, K. , A. Mogi, Y. Matsumoto, and T. Takita. 2005. Effect of caffeine on the body fat and lipid transfigurati on of rats fed on a high-fat diet. Biosci. , Biotech. Biochem. , 69 22192223. Li, J. , B. Tan, K. Mai, Q. Ai, W. Zhang, W. Xu, Z. Liufu, and H. Ma. 2006. Comparative study between probiotic bacterium Arthrobacter XE-7 and chloramphenicol on protection of Penaeus chinensis post-larvae from pathogenic vibrios. Aquaculture 253 140147.Lin, Z. B. and H. N. Zhang. 2004. Anti-tumor and immunoregulatory activities of Ganoderma lucidum and its potential mechanisms. Acta Pharmacologica Sinica 25 13871395. Mazzafera, P. 2002. Degratation of caffeine by microorganisms and potential use of decaffeinated coffee husk and pulp in animal feeding. Scientia Agricola 59, 815821. Meunpol, O. , K. Lopinyosiri, and P. Menasveta. 2003. The effects of ozone and probiotics on the survival of shadowy tiger wood pewee (Penaeus monodon). Aquaculture 220 437448. Moreau, Y. , J. L. Arredondo, I. Perraud-Gaime, and S. Roussos. 2003.Dietary utilisation of protein and energy from fresh and ensiled coffee pulp by the Nile tilapia Oreochromis niloticus. Brazil. Arch. Biol. Tech. , 46 223231. Pellegrini, N. , M. Sera? ni, B. Colombi, D. Del Rio, S. Salvatore, M. Bianchi, and F. Brighenti. 2003. follow antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different in vitro assays. J. Nut. , 133 28122819. Reitman, S. And S. Frankel. 1957. colorimetric determination of glutamic oxaloacetic and glutamic pyruvic transaminases.Am. J. Clin. Path. , 28 53-56. Serrano, F. S.J. , J. L. S. Lopez, and L. O. G. Martin. 1995. Caffeine quelling of glycogen phosphorylase from Mytilus galloprovincialis mantle tissue. Intern. J. Biochem. carrell Biol. , 27 911916. Tan, B. K. H. and J. Vanitha. 2004. Immunomodulatory and germicide effect of some traditional Chinese medicinal plants. Cur. Med. Chem. , 11 14231430. Teuber, M. 2001. Veterinary use and antibiotic resistance. Cur. Opin. Microbiol. , 4 493-499. Trinder, P. 1969. Determination of glucose concentration in the blood. Ann. Clin. Biochem. , 6 24. Ulloa, R. J. B. and J. A. J. Verreth. 2003.Growth of Oreochromis aureus fed with diets containing graded levels of coffee pulp and reared in two culture systems. Aquaculture 217 275283. Vaseeharan, B. and P. Ramasamy. 2003. Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Let. Appl. Microbiol. , 36 8387. Vinson, J. A. , K. Patel, and G. Agbor. 2005. Polyphenols total amounts in foods and beverages and US per capital consumption. In ACS 230th National Meeting. daybook of Abstracts (n. AGFD 10). American chemical substance Society, Washington.